Proteolytic processing of human brain alpha spectrin (fodrin): identification of a hypersensitive site
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چکیده
منابع مشابه
Proteolytic processing of human brain alpha spectrin (fodrin): identification of a hypersensitive site.
The processing of brain spectrin (fodrin) by calcium-dependent proteases at the postsynaptic membrane has been postulated to be one of the central molecular mechanisms underlying long-term potentiation (LTP). The effect of such processing on the structure and function of brain spectrin, and on spectrin's ability to organize or otherwise regulate receptor function remains unclear. To address the...
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Controlled digestion with o+chymotrypsin of inside-out vesicles from spectrin-depleted human erythrocyte membranes decreases by up to 90% the number of binding sites for [3’P]spectrin, but does not affect the K,) for [3ZPlspectrin of the sites which remain. Water-soluble polypeptides are released from the membranes during partial proteolysis with cY-chymotrypsin which are potent competitive inh...
متن کاملBrain spectrin (fodrin) interacts with phospholipids as revealed by intrinsic fluorescence quenching and monolayer experiments.
We demonstrate that phospholipid vesicles affect the intrinsic fluorescence of isolated brain spectrin. In the present studies we tested the effects of vesicles prepared from phosphatidylcholine (PtdCho) alone, in addition to vesicles containing PtdCho mixed with other phospholipids [phosphatidylethanolamine (PtdEtn) and phosphatidylserine] as well as from total lipid mixture extracted from bra...
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RATIONALE ABCA3 is a lipid transporter in the limiting membrane of lamellar bodies in alveolar type II cells. Mutations in the ABCA3 gene cause respiratory distress syndrome in new-borns and childhood interstitial lung disease. ABCA3 is N-terminally cleaved by an as yet unknown protease, a process believed to regulate ABCA3 activity. METHODS The exact site where ABCA3 is cleaved was localized...
متن کاملIdentification of three novel spectrin alpha I/74 mutations in hereditary elliptocytosis: further support for a triple-stranded folding unit model of the spectrin heterodimer contact site.
Six individuals with hereditary elliptocytosis (HE) or hereditary pyropoikilocytosis (HPP) from three unrelated families were evaluated. Defects in the ability of spectrin (Sp) to undergo self-association were present, and associated with increased recovery of the Sp alpha I 74-kD fragment after limited tryptic digestion (Sp alpha I/74 variant). Because mutations associated with the Sp alpha I/...
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ژورنال
عنوان ژورنال: The Journal of Neuroscience
سال: 1988
ISSN: 0270-6474,1529-2401
DOI: 10.1523/jneurosci.08-07-02640.1988